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fluoroplex tissue bath fluorometry system  (IonOptix)

 
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    IonOptix fluoroplex tissue bath fluorometry system
    Endosomolytic nanoplex delivery system enhances the delivery of p-HSP20 and inhibition of contraction. Rings of rat aorta were suspended in a <t>FluoroPlex</t> Tissue Bath Fluorometry System that can measure contraction and intracellular calcium concentration simultaneously. Rings were untreated (control), treated with different doses of the p-HSP20 peptide alone (100, 500, and 1000 μM), or peptide-nanoplex formulations (100, 200, and 500 μM), and inhibition of contraction and calcium concentration were measured. (A) Representative tracing showing contraction with PE and inhibition of contraction with p-HSP20 NP. (B) Cumulative data demonstrating enhanced inhibition of contraction with p-HSP20 or p-HSP20 NP. (C) Representative tracing showing inhibition of contraction and intracellular calcium concentration with 500 μM p-HSP20 NP (500 μM polyplex). (D) Cumulative data showing percent inhibition of contraction (force) and intracellular calcium concentration ([Ca2+]i). A fivefold increase in peptide mediated bioactivity was observed when delivered through the endosomolytic NP formulation compared to peptide alone without any change in the [Ca2+]i. *p<0.05, **p<0.001, ****p<0.00001, n = 4–6 (one-way ANOVA). p-HSP20 NP, p-HSP20 nanoplex.
    Fluoroplex Tissue Bath Fluorometry System, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroplex+tissue+bath+fluorometry+system/fluoroplex+tissue+bath+fluorometry+system/pmc06450456-265-10-15
    Average 90 stars, based on 1 article reviews
    fluoroplex tissue bath fluorometry system - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue"

    Article Title: Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2018.0169

    Endosomolytic nanoplex delivery system enhances the delivery of p-HSP20 and inhibition of contraction. Rings of rat aorta were suspended in a FluoroPlex Tissue Bath Fluorometry System that can measure contraction and intracellular calcium concentration simultaneously. Rings were untreated (control), treated with different doses of the p-HSP20 peptide alone (100, 500, and 1000 μM), or peptide-nanoplex formulations (100, 200, and 500 μM), and inhibition of contraction and calcium concentration were measured. (A) Representative tracing showing contraction with PE and inhibition of contraction with p-HSP20 NP. (B) Cumulative data demonstrating enhanced inhibition of contraction with p-HSP20 or p-HSP20 NP. (C) Representative tracing showing inhibition of contraction and intracellular calcium concentration with 500 μM p-HSP20 NP (500 μM polyplex). (D) Cumulative data showing percent inhibition of contraction (force) and intracellular calcium concentration ([Ca2+]i). A fivefold increase in peptide mediated bioactivity was observed when delivered through the endosomolytic NP formulation compared to peptide alone without any change in the [Ca2+]i. *p<0.05, **p<0.001, ****p<0.00001, n = 4–6 (one-way ANOVA). p-HSP20 NP, p-HSP20 nanoplex.
    Figure Legend Snippet: Endosomolytic nanoplex delivery system enhances the delivery of p-HSP20 and inhibition of contraction. Rings of rat aorta were suspended in a FluoroPlex Tissue Bath Fluorometry System that can measure contraction and intracellular calcium concentration simultaneously. Rings were untreated (control), treated with different doses of the p-HSP20 peptide alone (100, 500, and 1000 μM), or peptide-nanoplex formulations (100, 200, and 500 μM), and inhibition of contraction and calcium concentration were measured. (A) Representative tracing showing contraction with PE and inhibition of contraction with p-HSP20 NP. (B) Cumulative data demonstrating enhanced inhibition of contraction with p-HSP20 or p-HSP20 NP. (C) Representative tracing showing inhibition of contraction and intracellular calcium concentration with 500 μM p-HSP20 NP (500 μM polyplex). (D) Cumulative data showing percent inhibition of contraction (force) and intracellular calcium concentration ([Ca2+]i). A fivefold increase in peptide mediated bioactivity was observed when delivered through the endosomolytic NP formulation compared to peptide alone without any change in the [Ca2+]i. *p<0.05, **p<0.001, ****p<0.00001, n = 4–6 (one-way ANOVA). p-HSP20 NP, p-HSP20 nanoplex.

    Techniques Used: Inhibition, Concentration Assay

    Related Articles

    Fluorescence:

    Article Title: Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue
    Article Snippet: .. Cytosolic Ca2+measurements Rings of rat aorta were suspended on hooks in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Force measurements were obtained with a Radnoti force transducer (Radnoti Glass Technology Inc., Monrovia, CA) interfaced with Power Lab from AD Instruments (Colorado Springs, CO) as described earlier (21).

    Article Title: BRILLIANT BLUE FCF IS A NON-TOXIC DYE FOR SAPHENOUS VEIN GRAFT MARKING THAT ABROGATES RESPONSE TO INJURY
    Article Snippet: .. Measurement of purinergic receptor-induced contraction and cytosolic calcium ion flux Rat aorta was dissected free of fat and connective tissue, sectioned into 1-mm rings, and suspended in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Rings were loaded with 10 μM Fura-2 AM ester (Invitrogen, Carlsbad, CA) and 0.01% Pluronic F-127 (Invitrogen) in the bicarbonate buffer as described previously, 14 and then either left untreated or treated with FCF (50 μM), P2X 7 R antagonists periodate oxidized sodium salt (oATP; 50 μM), KN-62 (10 μM), or BBG (50 μM) for 30 min prior to contraction with the P2X 7 R agonist 2′ (3′)-O-(4-Benzoylbenzoyl)adenosine 5′-triphosphate (BzATP; 100 μM).

    Article Title: Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue
    Article Snippet: .. Rings of rat aorta were suspended on hooks in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology, Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. ..

    Article Title: Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue
    Article Snippet: .. Cytosolic Ca 2+ measurements Rings of rat aorta were suspended on hooks in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology, Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Force measurements were obtained with a Radnoti force transducer (Radnoti Glass Technology, Inc.) interfaced with Power Lab from AD Instruments (Colorado Springs) as described earlier.

    Article Title: Role of Cyclic Nucleotide-Dependent Actin Cytoskeletal Dynamics: [Ca 2+ ] i and Force Suppression in Forskolin-Pretreated Porcine Coronary Arteries
    Article Snippet: .. Rings of PCA were suspended on hooks in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Force measurements were obtained with a Radnoti force transducer (Radnoti Glass Technology Inc., Monrovia, CA) interfaced with Power Lab from AD Instruments (Colorado Springs, CO).

    Article Title: Role of cyclic nucleotide-dependent actin cytoskeletal dynamics:Ca(2+)](i) and force suppression in forskolin-pretreated porcine coronary arteries.
    Article Snippet: .. Cytosolic [Ca2+]i Measurements Rings of PCA were suspended on hooks in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Force measurements were obtained with a Radnoti force transducer (Radnoti Glass Technology Inc., Monrovia, CA) interfaced with Power Lab from AD Instruments (Colorado Springs, CO).

    Article Title: BRILLIANT BLUE FCF IS A NON-TOXIC DYE FOR SAPHENOUS VEIN GRAFT MARKING THAT ABROGATES RESPONSE TO INJURY
    Article Snippet: .. Rat aorta was dissected free of fat and connective tissue, sectioned into 1-mm rings, and suspended in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Rings were loaded with 10 μM Fura-2 AM ester (Invitrogen, Carlsbad, CA) and 0.01% Pluronic F-127 (Invitrogen) in the bicarbonate buffer as described previously, 14 and then either left untreated or treated with FCF (50 μM), P2X 7 R antagonists periodate oxidized sodium salt (oATP; 50 μM), KN-62 (10 μM), or BBG (50 μM) for 30 min prior to contraction with the P2X 7 R agonist 2′ (3′)-O-(4-Benzoylbenzoyl)adenosine 5′-triphosphate (BzATP; 100 μM).

    Tissue:

    Article Title: BRILLIANT BLUE FCF IS A NON-TOXIC DYE FOR SAPHENOUS VEIN GRAFT MARKING THAT ABROGATES RESPONSE TO INJURY
    Article Snippet: .. Measurement of purinergic receptor-induced contraction and cytosolic calcium ion flux Rat aorta was dissected free of fat and connective tissue, sectioned into 1-mm rings, and suspended in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Rings were loaded with 10 μM Fura-2 AM ester (Invitrogen, Carlsbad, CA) and 0.01% Pluronic F-127 (Invitrogen) in the bicarbonate buffer as described previously, 14 and then either left untreated or treated with FCF (50 μM), P2X 7 R antagonists periodate oxidized sodium salt (oATP; 50 μM), KN-62 (10 μM), or BBG (50 μM) for 30 min prior to contraction with the P2X 7 R agonist 2′ (3′)-O-(4-Benzoylbenzoyl)adenosine 5′-triphosphate (BzATP; 100 μM).

    Article Title: BRILLIANT BLUE FCF IS A NON-TOXIC DYE FOR SAPHENOUS VEIN GRAFT MARKING THAT ABROGATES RESPONSE TO INJURY
    Article Snippet: .. Rat aorta was dissected free of fat and connective tissue, sectioned into 1-mm rings, and suspended in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement. .. Rings were loaded with 10 μM Fura-2 AM ester (Invitrogen, Carlsbad, CA) and 0.01% Pluronic F-127 (Invitrogen) in the bicarbonate buffer as described previously, 14 and then either left untreated or treated with FCF (50 μM), P2X 7 R antagonists periodate oxidized sodium salt (oATP; 50 μM), KN-62 (10 μM), or BBG (50 μM) for 30 min prior to contraction with the P2X 7 R agonist 2′ (3′)-O-(4-Benzoylbenzoyl)adenosine 5′-triphosphate (BzATP; 100 μM).



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    Endosomolytic nanoplex delivery system enhances the delivery of p-HSP20 and inhibition of contraction. Rings of rat aorta were suspended in a <t>FluoroPlex</t> Tissue Bath Fluorometry System that can measure contraction and intracellular calcium concentration simultaneously. Rings were untreated (control), treated with different doses of the p-HSP20 peptide alone (100, 500, and 1000 μM), or peptide-nanoplex formulations (100, 200, and 500 μM), and inhibition of contraction and calcium concentration were measured. (A) Representative tracing showing contraction with PE and inhibition of contraction with p-HSP20 NP. (B) Cumulative data demonstrating enhanced inhibition of contraction with p-HSP20 or p-HSP20 NP. (C) Representative tracing showing inhibition of contraction and intracellular calcium concentration with 500 μM p-HSP20 NP (500 μM polyplex). (D) Cumulative data showing percent inhibition of contraction (force) and intracellular calcium concentration ([Ca2+]i). A fivefold increase in peptide mediated bioactivity was observed when delivered through the endosomolytic NP formulation compared to peptide alone without any change in the [Ca2+]i. *p<0.05, **p<0.001, ****p<0.00001, n = 4–6 (one-way ANOVA). p-HSP20 NP, p-HSP20 nanoplex.
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    Image Search Results


    Endosomolytic nanoplex delivery system enhances the delivery of p-HSP20 and inhibition of contraction. Rings of rat aorta were suspended in a FluoroPlex Tissue Bath Fluorometry System that can measure contraction and intracellular calcium concentration simultaneously. Rings were untreated (control), treated with different doses of the p-HSP20 peptide alone (100, 500, and 1000 μM), or peptide-nanoplex formulations (100, 200, and 500 μM), and inhibition of contraction and calcium concentration were measured. (A) Representative tracing showing contraction with PE and inhibition of contraction with p-HSP20 NP. (B) Cumulative data demonstrating enhanced inhibition of contraction with p-HSP20 or p-HSP20 NP. (C) Representative tracing showing inhibition of contraction and intracellular calcium concentration with 500 μM p-HSP20 NP (500 μM polyplex). (D) Cumulative data showing percent inhibition of contraction (force) and intracellular calcium concentration ([Ca2+]i). A fivefold increase in peptide mediated bioactivity was observed when delivered through the endosomolytic NP formulation compared to peptide alone without any change in the [Ca2+]i. *p<0.05, **p<0.001, ****p<0.00001, n = 4–6 (one-way ANOVA). p-HSP20 NP, p-HSP20 nanoplex.

    Journal: Tissue Engineering. Part A

    Article Title: Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue

    doi: 10.1089/ten.tea.2018.0169

    Figure Lengend Snippet: Endosomolytic nanoplex delivery system enhances the delivery of p-HSP20 and inhibition of contraction. Rings of rat aorta were suspended in a FluoroPlex Tissue Bath Fluorometry System that can measure contraction and intracellular calcium concentration simultaneously. Rings were untreated (control), treated with different doses of the p-HSP20 peptide alone (100, 500, and 1000 μM), or peptide-nanoplex formulations (100, 200, and 500 μM), and inhibition of contraction and calcium concentration were measured. (A) Representative tracing showing contraction with PE and inhibition of contraction with p-HSP20 NP. (B) Cumulative data demonstrating enhanced inhibition of contraction with p-HSP20 or p-HSP20 NP. (C) Representative tracing showing inhibition of contraction and intracellular calcium concentration with 500 μM p-HSP20 NP (500 μM polyplex). (D) Cumulative data showing percent inhibition of contraction (force) and intracellular calcium concentration ([Ca2+]i). A fivefold increase in peptide mediated bioactivity was observed when delivered through the endosomolytic NP formulation compared to peptide alone without any change in the [Ca2+]i. *p<0.05, **p<0.001, ****p<0.00001, n = 4–6 (one-way ANOVA). p-HSP20 NP, p-HSP20 nanoplex.

    Article Snippet: Rings of rat aorta were suspended on hooks in a FluoroPlex Tissue Bath Fluorometry System (IonOptix LLC, Milton, MA and Radnoti Glass Technology, Inc., Monrovia, CA), which enables fluorescence ion recording in parallel with force measurement.

    Techniques: Inhibition, Concentration Assay